SILAC – Neural Basal Medium 0428

SILAC media is arranged explicitly for marking tests including the utilization of stable amino corrosive isotopes. Athena gives a few of the most regularly utilized media without L-arginine, L-lysine, L-leucine and L-methionine, the four for the most part generally utilized weighty isotope marked amino acids.

Powdered Formulations

The SILAC fixings are processed to a fine molecule size, mixed to give a homogenous blend and filled in dampness confirmation pockets. Each pocket contains sufficient medium to get ready 1 liter of medium.
Savvy
60% more affordable than fluid
Lower delivering costs
long term time span of usability
Stock arrangements of every one of the four missing amino acids can be bought independently.

Prepared to-Use Liquid

The SILAC medium is ready as a prepared to-utilize fluid that is sterile endlessly filled in 500 ml bottles. Each jug is provided with stock arrangements of every one of the four missing amino acids. This grants reconstitution of complete medium.
Advantageous prepared to-utilize Kills planning time
Simply add clean amino acids and enhancements.

SILAC media is arranged explicitly for naming tests including the utilization
of stable amino corrosive isotopes. Athena gives a few of the most normally
utilized media without L-arginine, L-lysine, L-leucine and L-methionine, the four
generally normally utilized weighty isotope marked amino acids.

Powdered Formulations

The SILAC fixings are processed to a fine molecule size, mixed to give a homogenous blend and filled in dampness verification pockets. Each pocket contains enough medium to plan 1 liter of medium.
● Savvy – 60% more affordable than fluid
● Lower delivering costs
● long term timeframe of realistic usability
Stock arrangements of every one of the four missing amino acids can be bought independently.

Prepared to-Use Liquid

The SILAC medium is ready as a prepared to-utilize fluid that is clean filled and
filled in 500 ml boles. Each bole is provided with stock arrangements of each of the
four missing amino acids. This licenses reconstitution of complete medium.
● Advantageous prepared to-utilize
● Takes out planning time
● Simply add clean amino acids and enhancements
Custom Media
Assuming that you want a medium detailing without other amino acids or parts,
AthenaES spends significant time in low volume custom creation of plans of
other cell culture media. To demand a custom tissue culture go to Custom Medium Reqest to present a web-based demand structure.

Details and Storage
Capacity 4°C
Security at 4°C Liquid: 3 – 4 months
Powder: 3 years
pH 7.3 ± 0.2
Osmolality 270 – 300 mOsM

Requesting Information:

Feline. No. Depiction Size
0420 DMEM High Glucose 500mL
0421 DMEM Low Glucose 500mL
0422 RPMI 1640 500mL
0423 DMEM/F12 500mL
0424 MEM 500mL
0425 Ham’s F12 500mL
0426 McCoy’s 5A 500mL
0428 Neural Basal Medium 500ml
0429 Insect SFM 500ml
0430 DMEM High Glucose 1L, powder
0431 DMEM Low Glucose 1L, powder
0432 RPMI 1640 1L, powder
0433 DMEM/F12 1L, powder
0416 1,149 mM L-Arg 6 x 0.5mL
0417 800 mM L-Lys 6 x 0.5ml
0418 160 mM L-Leu 12 x 1.25ml
0419 201 mM L-Met 6 x 0.5ml
All media are made without Phenol Red or Sodium Pyruvate as well as without Arg, Lys, Leu,Met.

Unique

Refined essential neurons are a deep rooted model for the investigation of neuronal capacity. Ordinary stable isotope naming with amino acids in cell culture (SILAC) requires almost complete metabolic marking of proteins and in this way is hard to apply to refined essential neurons, which don’t isolate in culture. Here we depict a convention that uses a multiplex SILAC marking procedure for essential refined neurons. In this methodology, two unique arrangements of weighty amino acids are utilized for naming cells for the different exploratory circumstances. This considers clear SILAC quantitation utilizing somewhat named cells in light of the fact that the two cell populaces are similarly named 100% of the time.

Presentation

Stable isotope marking with amino acids in cell culture (SILAC) includes cell culture in media containing “light” (normal) or “weighty” isotope-containing amino acids (1). It is vital to get a serious level of mark fuse on the grounds that fragmented naming will slant the SILAC proportion for the light protein (2). To guarantee almost complete marking, it is by and large expected to keep up with cells in SILAC media for somewhere around five cell divisions so that even proteins with zero turnover rate will be exceptionally named (>97%) by weakening alone (1,3).

Essential neurons are broadly utilized as a vital model in neuroscience since overall their useful properties more intently look like the in vivo state than those of changed cell societies. Since the essential neurons don’t separate in culture, it is challenging to utilize the standard light/weighty SILAC marking in view of the issue of fragmented naming.

To conquer this trouble, a technique has been accounted for in which the SILAC proportion is rectified for deficient naming by checking the name consolidation of each protein (4,5). In any case, this system has a few clear drawbacks. To start with, each SILAC investigation requires an equal examination to gauge the name fuse for each and every protein.

Notwithstanding the additional expense and exertion, it is hard to get the name fuse for each protein since it requires the protein be measured in two investigations. An impressive extent of the SILAC protein proportions can’t be remedied in light of the fact that for investigation of mind boggling protein blends by fluid chromatography couple mass spectrometry (LC MS/MS), protein distinguishing proof is normally just to some degree covered between imitate examinations (2). Besides, the adjustment step presents extra variety that compromises the exactness of quantitation.

To bypass these issues, we utilize a multiplex SILAC naming technique for essential neurons (Fig. 1) (6-8). Rather than utilizing light and weighty naming amino acids to recognize the two trial conditions, we utilize two distinct arrangements of weighty amino acids, D4-lysine/13C6-arginine (Lys4/Arg6) and 13C615N2-lysine/13C615N4-arginine (Lys8/Arg10).

DKW Basal Salt Mixture

CP007-010 GenDepot 10X1L 99 EUR

DKW Basal Salt Mixture

CP007-500 GenDepot 50L 126 EUR

Anderson's Basal Salt

CP001-010 GenDepot 10x1L 99 EUR

Anderson's Basal Salt

CP001-500 GenDepot 50L 126 EUR

Anderson's Basal Salt

CP002-010 GenDepot 10X1L 99 EUR

Anderson's Basal Salt

CP002-500 GenDepot 50L 126 EUR

Ethyl sulfate, sodium salt, 2 mg

0902-2 AthenaES 2 mg 69 EUR

Ethyl sulfate-d5, sodium salt, 2 mg

0902D-2 AthenaES 2 mg 92 EUR

D-Luciferin, potassium salt

10101-2 Biotium 1G 714 EUR

D-Luciferin, sodium salt

10102-2 Biotium 1G 714 EUR

Eplivanserin (mixture)

HY-10792A MedChemExpress 5mg 165 EUR

2-Mercaptopyridine-N-oxide, sodium salt

20-abx180003 Abbexa
  • 244.00 EUR
  • 203.00 EUR
  • 398.00 EUR
  • 100 g
  • 25 g
  • 500 g

Universal qPCR Mixture

QPCR1000-UNIV Bio Basic 4X1.25ml 276.2 EUR

NTP Mixture (10mM)

ND0056 Bio Basic 0.5ml 91.76 EUR

NTP Mixture (25mM)

ND0057 Bio Basic 0.5ml 154.4 EUR

Plant Preservative Mixture

PCT01 Plant Cell Technologies 30 ml 90 EUR

Plant Preservative Mixture

PCT02 Plant Cell Technologies 100 ml 749 EUR

Plant Preservative Mixture

PCT03 Plant Cell Technologies 250 ml 310 EUR

Plant Preservative Mixture

PCT04 Plant Cell Technologies 500 ml 450 EUR

Since the two cell populaces consolidate the two arrangements of amino acids at similar rate, they are similarly marked all of the time. SILAC quantitation is finished utilizing the signs of the medium (Lys4/Arg6) and weighty (Lys8/Arg10) named peptides, and the unlabeled peptides can be overlooked. This takes into account clear and exact SILAC quantitation utilizing somewhat marked cells.

Leave a Comment