Homogeneous Immunoassay

Lumigen SPARCL is a homogeneous vicinity measure innovation using streak chemiluminescence location without strong help or wash steps taking into account examines to be finished in under 30 minutes. The SPARCL innovation empowers examines to be scaled down for high throughput screening while at the same time keeping up with touchy outcomes with great powerful reach. SPARCL can be mechanized and adjusted for a long time including ELISA, protein-protein and protein-nucleic corrosive cooperations, and high throughput restricting measures.

Complete examines in under 30 minutes – Save time with just a single brooding advance, no partition or wash steps and immediate sign age from streak chemiluminescence
Savvy – Use less reagents and instruments with no requirement for plate/dot covering activities, robotized washing stations or other costly identification gear.
Adaptable examine designs – Adaptable for various sorts of tests in arrangement or strong stage organizations to concentrate on a wide assortment of targets
Produce less waste – No wash implies no removal of wash arrangement. SPARCL creates just 1x well volume of waste contrasted with 13x well volume for traditional ELISA measures.
SPARCL Formats
Arrangement or strong stage examine
SPARCL can be executed in designs regardless of a strong stage. While utilizing a strong stage, both the acridan compound and a particular catch immunizer are coupled to strong stages like miniature particles or microtiter plates, though when the strong stage is overlooked the catch counter acting agent is straightforwardly marked with the acridan compound. The arrangement stage design kills the requirement for plate or molecule covering, further develops energy to abbreviate hatching time and because of the absence of a strong/arrangement interface offers a more local organic climate.

SPARCL Technology

SPARCL (Spatial Proximity Analyte Reagent Capture Luminescence) is a closeness subordinate chemiluminescent innovation for the location of explicit restricting connection or relationship between two restricting accomplices. In a SPARCL test, a limiting accomplice marked with a chemiluminescent substrate (acridan) and a subsequent restricting accomplice named with horseradish peroxidase (HRP) are carried into closeness to one another through a particular restricting occasion. As a result of this nearness of acridan to the HRP chemical a blaze of chemiluminescence is created upon expansion of a trigger arrangement containing hydrogen peroxide and an enhancer.

SPARCL A Simpler and Faster ELISA Alternative

SPARCL (Spatial Proximity Analyte Reagent Capture Luminescence) measures utilize two explicit antibodies; one formed to HRP, the other to acridan, a chemiluminescent substrate. Whenever HRP and acridan formed antibodies tie to their objective biomarker they are brought into nearness.

Endless supply of hydrogen peroxide (trigger arrangement), HRP catalyzes oxidation of proximal acridan particles causing a glimmer of chemiluminescence that is relative to biomarker fixation. Acridan atoms far off from HRP are not oxidized and accordingly produce no glow. This standard permits advancement of a scope of quick and delicate homogeneous immunoassays.