VIAGEN DirectPCR Lysis Reagent (Mouse Tail)

Viagen DirectPCR DNA Extraction System is a solitary cylinder framework for quick readiness of DNA from mouse tails, ear pieces, yolk sac, and culture cells. The patent-forthcoming parts created by researchers at Viagen Biotech Inc. permit the subsequent DNA concentrates to be viable with genomic PCR for genotyping. Rough concentrates of natural examples are not viable with numerous sub-atomic science grade responses, for example, polymerase chain response (PCR), partially because of inhibitors contained in unrefined concentrates. The DirectPCR reagents not just intervene the fast lysis of natural examples yet additionally contain inhibitors that successfully stifle the inhibitory exercises of rough lysates for PCR intensification, while maximally keeping up with the honesty of delivered genomic DNA. The patent-forthcoming basic strategy totally take out any arrangement move or cylinder opening advances, furnishing you with substancial additional time.

Brief system
1. Lyse tails in DirectPCR® Lysis Reagent.
2. Brood for 45 min at 85°C.
3. PCR genotyping with 1 μl lysates.

Definite conventions: Tail, Ear, Yolk Sac, and Cultured cells.

DirectPCR framework offers benefits over ordinary conventions that include:
· Efficient: Less involved time. Rough tail lysates for PCR!
· Cash saving: Cost-compelling responses.
· Safe: No natural reagents.
· Ecological: Less waste (natural reagents, tubes, tips, etc…)
· Dependable and proficient: Virtually 100 percent achievement rate with exceptional returns.

DirectPCR Lysis Reagent (mouse tail)

101-T 50ml (250 mouse tails).

102-T 100ml (500 mouse tails).

Viagen Biotech Inc.

Viagen Biotech Inc. is a main supplier of creative DNA extraction innovations and items for life science research. Viagen DirectPCR® DNA Extraction System is a solitary cylinder framework for quick arrangement of DNA from mouse tails, ear pieces, yolk sac, and culture cells. The patent-forthcoming parts created by researchers at Viagen Biotech Inc. permit the subsequent DNA concentrates to be viable with genomic PCR for genotyping. Unrefined concentrates of natural examples are not viable with numerous atomic science grade responses, for example, polymerase chain response (PCR), to some extent because of inhibitors contained in rough concentrates. The DirectPCR reagents not just intervene the fast lysis of natural examples yet additionally contain inhibitors that successfully smother the inhibitory exercises of rough lysates for PCR intensification, while maximally keeping up with the respectability of delivered genomic DNA. The system totally dispenses with any arrangement move or cylinder opening advances, saving a significant measure of time and exertion. DirectPCR reagents cost just 26 pennies for every tail, giving reserve funds at any utilization volume.

We additionally offer the most reasonable DNA markers contrasted with other driving organizations. We will keep on growing our abilities by effectively looking for examination and business organization with both scholastic foundations and industry trailblazers to foster new exploration devices. Our items are offered to scholarly research markets, biotechnology organizations, and drug organizations. Through a worldwide organization of in excess of 30 global wholesalers, we offer our quality items and administrations to an overall organization of exploration researchers in life science.

Viagen Biotech IncSupplier Diversity Partner DIR PCR LYSIS RGNT(MOUSE TAIL)

DirectPCR Lysis Reagent (Mouse Tail) 100ml (Up to 500 Tails). The framework is a solitary cylinder framework for quick planning of DNA from mouse tails. The patent-forthcoming parts permit the subsequent DNA concentrates to be viable with genomic PCR for genotyping. Rough concentrates of organic examples are not viable with numerous atomic science grade responses, for example, polymerase chain response (PCR), to some degree because of inhibitors contained in unrefined concentrates. The DirectPCR reagents not just intercede the fast lysis of organic examples yet additionally contain inhibitors that successfully smother the inhibitory exercises of rough lysates for PCR enhancement, while maximally keeping up with the respectability of delivered genomic DNA. The patent-forthcoming basic technique totally kills any arrangement move or cylinder opening advances, giving you significant additional time. Stable for quite a long time at 4Deg C/Ships at room temperature.

DirectPCR Lysis Reagent Tail

Portrayal:
DirectPCR® Lysis Reagent Tail (Patent Pending) was particularly created for the lysis of mouse tail tissue.
After a concise hotness treatment, the unrefined lysates are straightforwardly utilized for PCR without tedious genomic
DNA segregation. Particular DirectPCR® items are accessible for the lysis of mouse ear, yolk sac or
refined mammalian cells.

Request Information:

DirectPCR-Tail for up to 250 mouse tails (50 ml) request no. 31-101-T
DirectPCR-Tail for up to 500 mouse tails (100 ml) request no. 31-102-T
Capacity:
Store DirectPCR®-Tail safeguarded from light at 4 – 8 °C. The reagent is steady something like a half year from the
date of shipment under these circumstances. Precious stones might shape upon shipment or capacity and ought to be
broken down by short warming to 37 °C before use.

Convention (see TABLE 1):

1. Add 200 – 300 μl DirectPCR®-Tail containing newly pre-arranged 0.2-0.4 mg/ml Proteinase K (for example
peqGOLD Proteinase K arrangement [20 mg/ml], request no. 04-1075) to a 0.5 cm mouse tail piece.
Turn the cylinder in a pivoting hybridization stove at 55 °C for 3 – 16 hours until complete lysis is
accomplished.
NOTE: Although 200 μl DirectPCR- Tail is generally adequate for complete lysis of 0.5 cm tail, use of
300 μl typically yields more reproducible outcomes in view of better blending proficiency. It is prescribed to
analyze a few unique measures of DirectPCR® – Tail reagents in the underlying preliminaries. For limited quantities of
tissue, DirectPCR reagent should be weakened with water, and measure of lysate for PCR responses should be
expanded relatively. SEE TABLE 1.
NOTE: Proteinase K is possibly steady for as long as 24 hours when added to DirectPCR reagent. Continuously use newly
arranged Proteinase K.
NOTE: Rotating hybridization broiler by and large performs better compared to shaking plate. Utilize 0.75 ml tubes for less than 200 μl of DirectPCR® – Tail. Since the lysates are utilized for PCR without DNA seclusion, DNA fracture by delayed pivot won’t essentially impact PCR execution. Utilize generally corresponding volume of
DirectPCR – Tail for various scaled examples. SEE TABLE 1.
2. Hatch rough lysates at 85 °C for 45 min by drifting the entire rack (containing tubes) on a water
shower to accomplish total hotness inactivation of Proteinase K.
Axis flotsam and jetsam or consolidated water for 10 sec before stage 3 (discretionary).
NOTE: Crude lysates might be put away for 1 year at – 20 °C or for multi week at 4 °C without losing proficiency.
3. Straightforwardly utilize 0.5 – 1 μl of lysate per 50 μl genotyping PCR response. (see TABLE 1)
[4. Discretionary: DNA in rough lysates can be proficiently protected for additional cleansing. Add NaCl to afinal centralization of 250 mM, and 0.7 volumes of isopropanol. DNA will emerge from an
fluid stage. Rotator at 4 °C for 2 min to accelerate DNA. Dispose of supernatant. Wash DNA with 1 ml 70 % EtOH and disintegrate pellet in 50 μl 10 mM Tris-HCl (8.0). Utilize 1 μl for PCR.]

Application notes

1. Complete Lysis: Mouse tail pieces ought not be longer than 0.5 cm and should be totally
submerged in lysis reagent. No huge tissue parts ought to be noticeable after lysis.
2. Proteinase K Inactivation: Inactivation of Proteinase K is accomplished by brooding at 85 °C for 45 min.
This progression is fundamental to stay away from processing of Taq Polymerase by Proteinase K in PCR response, don’t
skirt this progression!
3. Measure of format: Use as little lysate for PCR response as could be expected, as higher centralizations of
DirectPCR reagent could repress PCR response. (See TABLE 1).
4. Cylinders and dissipation: If response volume is under 200 μl during lysis, utilize 0.75 ml response tubes
to restrict dissipation.

DIRECTPCR LYSIS REAGENT (TAIL)
102-T	Viagen	100ml	248.4 EUR
DIRECTPCR LYSIS REAGENT (EAR)
401-E	Viagen	25 ml	169.2 EUR
DIRECTPCR LYSIS REAGENT (EAR)
402-E	Viagen	50 ml	248.4 EUR
DIRECTPCR LYSIS REAGENT (CELL)
301-C	Viagen	50 ml	169.2 EUR
DIRECTPCR LYSIS REAGENT (CELL)
302-C	Viagen	100 ml	248.4 EUR
DIRECTPCR LYSIS RGNT(YOLK SAC)
201-Y	Viagen	50 ml	169.2 EUR
DIRECTPCR LYSIS YOLK SAC 100ML
202-Y	Viagen	100 ml	248.4 EUR
DIRECTPCR LYSIS REAGENT (TAIL)
101-T	Cusabio	50 ml	169.2 EUR
Xpro IP Cell Lysis Reagent,SDS Free
X3100-010	GenDepot	100ml	208.8 EUR
Xpro IP Cell Lysis Reagent,SDS Free
X3100-050	GenDepot	500ml	380.4 EUR
PROTEINASE K SOLUTION
501-PK	Viagen	5 ml	182.4 EUR
PROTEINASE K SOLUTION 10ML
502-PK	Viagen	10 ml	286.8 EUR
PROTEINASE K SOLUTION 25ML
503-PK	Viagen	25 ml	602.4 EUR
Lysis Buffer
abx098984-LysisBuffer120ml	Abbexa	Lysis Buffer 1 (20 ml)	184.8 EUR
Lysis Buffer
abx098984-LysisBuffer3100ml	Abbexa	Lysis Buffer 3 (100ml)	276 EUR

5. Little example volume and weakening: If limited quantities of tissue are to be lysed, weaken lysis reagent with
water and increment measure of lysate for PCR response relatively. (see TABLE 1)
6. Investigating: If PCR results don’t match assumptions, perform positiv control with detached
(unadulterated) genomic DNA, to avoid issues with the warm cycler, PCR convention, PCR reagents or
response technique.