SensiFast SYBR No-ROX Kit


Excellent reproducibility, sensitivity, and robustness of the DNA and RNA template assay, under fast thermal cycling conditions.

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  • Reproducible: consistent results between technical replicates for greater confidence in the results.
  • Specific – Antibody-mediated hot-start DNA polymerase minimizes non-specific amplification to improve assay sensitivity and reliability
  • Sensitive – Reliable quantification of low abundance targets and sparse samples
  • Robust – Accurate and reliable detection of DNA and RNA targets from a wide range of sample types
  • Fast – delivers accurate and reproducible analysis results in as little as 30 minutes.

Product description

The SensiFAST ™ SYBR No-ROX Kit has been developed for fast, high-precision real-time PCR and has been validated on all commonly used real-time instruments that do not require the passive ROX reference dye.

A combination of the latest advancements in PCR buffers and enhancer chemistry ensures that the SensiFAST SYBR No-ROX Kit produces reliable assay results under fast thermal cycling conditions. An antibody-mediated hot-start DNA polymerase system promotes highly specific amplification, which in turn improves the sensitivity and dynamic range of the assay.

The SensiFAST SYBR No-ROX Kit has been optimized for optimal performance in conjunction with the SensiFAST cDNA Synthesis Kit, which offers fast and unbiased cDNA synthesis, without compromising cDNA performance or coverage.


  1. Gene expression analysis
  2. DNA / RNA target detection
  3. miRNA profiling/quantification
  4. Copy number variation analysis (CNV)

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